Taq polymerase glycerol-free is a lyophilization-compatible version of highly purified recombinant standard PCR enzyme. Host DNAs are removed almost entirely from the enzyme to minimize false positive reactions during molecular diagnostic applications. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs.   

Application  

• Routine PCR and RT-PCR 
• Conventional and Real-Time PCR
• PCR for molecular diagnostics
• Manufacture of dried amplification mixtures

Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity

Temperature optimum: Approximately +75°C

Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween-20

Purity: ≥95% (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) 

RNase contamination test: Not detectable (Incubation with 40U enzyme and human total RNA at 37°C, 1hr)

DNA contamination test: [E.coli DNA] less than 1 copy/5U enzyme, [Human DNA] Not detectable

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20°C.