Premium-Taq polymerase provides utmost specific amplification by a dual system; antibody-mediated hot start polymerase and PMT (Polymerase Modulator on Temperature)-applied buffer. Anti-Taq antibody inhibits the Taq polymerase activity until it reaches to denaturation temperature and PMT-applied buffer reduces the primer-dimer formation and nonspecific amplification during the PCR cycles. The 5x reaction mix supplied together contains pH-buffering agent, salts, magnesium, dNTPs, and PMT additive. 

Application 

• Antibody-mediated Hot-start PCR
• High specific amplification and minimize the primer dimer formation
• Fast PCR assays with a short enzyme activation time and fast cycling
• Multiplex PCR
• PCR for molecular diagnostics

Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity

Enzyme activation: 2 min at +95°C

Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol

DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) 

RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20°C