One-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Hot-Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl₂, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP.  

Application 

• Viral RNA detection
• Gene-expression analysis
• Molecular Diagnosis
• Endpoint one-step RT-PCR from any RNA transcript

Figure 1. Multiplex One-step RT-PCR of up to 4 targets by One-Step RT-PCR Kit [Hot-Taq]. Multiplex one-step RT-PCR in single-tube reaction was performed with total RNA extracted from hot pepper plant (Capsicum annuum) and by adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The primer sets for the followed genes were used. CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), Cadhn (761 bp).

Properties: Conventional RT-PCR, Hot-start

Heat activation: +95°C for 15 minutes after reverse transcription reaction.

Activity test: Performance test in PCR (Human total RNA, Actin fragment): Corresponds to specification

Stability: 12 months at -20°C.