qRT-PCR Kit [v4] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2 causing the new Coronavirus infectious disease (COVID-19). The kit performance was confirmed using the materials and methods approved by WHO on 17 Jan, 2020. The Enzyme Mix contains in this kit, is an optimized blend of Reverse Transcriptase, chemically-modified hot-start PCR enzyme, RNase inhibitor protein and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for the COVID-19 molecular diagnosis. In particular, carryover contaminated PCR products could be removed by the applied UDG system.

Application 

• Quantification of target RNA by real-time RT-PCR

Figure. Multiplex qRT-PCR using the RealHelix™ qRT-PCR Kit[v4] UDG System. Purified SARS-CoV-2 viral RNA(dilution fold; 10^-4, 10^-5, 10^-6,10^-7) or human total RNA(10, 1, 0.1, 0.01ng) were analyzed using three different sets of target-specific primers and probes(ORF8 and N gene from SARS-CoV-2 / RNase P gene from human). The probes were labeled with HEX(ORF8), Texas Red(N gene), or Cy5(RNase P). The multiplex reaction was tested using the RealHelix™ qRT-PCR Kit [v4] UDG System on a real-time PCR instrument, Bio-Rad CFX96.