PureHelix™ Fast-n-Pure Plasmid Kit is a simplified and fast kit than the conventional method for isolation of pure plasmids up to 20 ug for routine molecular biological applications, such as PCR, cloning, sequencing, in vitro transcription, transfection experiments, etc. PureHelix™ Fast-n-Pure Plasmid Kit is based on alkaline-lysis method and the binding properties of DNA on silica-gel membrane to provide the high-quality and high-yield plasmid DNAs. Especially, PureHelix™ Fast-n-Pure Plasmid Kit is also excellent to isolate low-copy plasmids and large size of circular DNAs, such as fosmids and recombinant BAC plasmids.

 

Figure 1. Isolation of large size plasmid using PureHelix™ Fast-n-Pure Plasmid kit.  

Figure 2. Isolation of plasmid DNA of high-copy, low-copy using PureHelix™ Fast-n-Pure Plasmid kit. 

Figure 3. Complete restriction digestion of the purified plasmid using PureHelix™ Fast-n-Pure Plasmid kit. Restriction enzymatic analysis of pUC19 DNA purified using PureHelix™ Fast-n-Pure Plasmid kit 1 : Isolated Uncut pUC19, 2 : Digested pUC19 with BamHI, 2 : Digested pUC19 with TaqI, 3 : Digested pUC19 with XbaI. 1 : Intact pUC19, 2 : BanHI cut, 3 : TaqI cut, 4 : XbaI cut 

Figure 4. Sequence analysis for plasmid DNA isolated by PureHelix™ Fast-n-Pure Plasmid Kit.

Application 

• Molecular biological application 
• Automatic fluorescent sequencing 
• Restriction enzymatic digestion and cloning
• Transfection of robust cells 

Quality Control

PureHelix™ Fast-n-Pure Plasmid Kit was tested for the isolation of high- or low-copy plasmids and fosmid DNA from E.coli cells containing each plasmid DNA. Also, the high-copy plasmid DNA isolated with PureHelix™ Fast-n-Pure Plasmid Kit was tested in the restriction-enzymatic digestion and the sequencing analysis.