HelixAmp™ DNA-free Taq polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene and purified using a process that minimizes level of contaminating host DNA. HelixAmp™ DNA-free Taq polymerase is high quality DNA polymerase and suitable for molecular diagnostics. Because the contaminating host genomic DNA can cause the false positive results, use of DNA-free Taq polymerase is very important in PCR detection of bacteria with universal primers(e.g. 16s rRNA specific primers). A chemically modified hot-start version of this enzyme, HelixAmp™ DNA-free Hot-Taq polymerase, is also available. (see the product list) 

Figure 1. HelixAmp™ DNA-free Taq polymerase was compared to Taq polymerse from competitors for performance and gDNA contamination by qPCR. HelixAmp™ DNA-free Taq (1.25U) was tested for presence of E. coli gDNA contamination in qPCR using 16s rRNA primers. 

Figure 2. Comparison of conventional Taq DNA polymerase and HelixAmp™ DNA-free Taq polymerase for the contamination of host DNA. PCRs were performed with or without template. Primers are specific for the E. coli 16s rRNA-coding gene. 

Figure 3. Comtamination test of host DNA in conventional Taq DNA polymerase(hot-start version) and HelixAmp™ DNA-free Taq polymerase(hot-start version) using a real-time PCR assay.

Contents 

• DNA-free Taq polymerase (5 units/㎕)
• 10X Reaction Buffer
• dNTP Mix (each 10 mM)
• 5X TuneUp™ solution 

Quality Control 

• Contamination assay for nucleases
• Contamination assay for host genomic DNA
• Activity and sensitivity assay 

Storage buffer 

• 50% Glycerol,20 mM Tris-HCl (pH9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1 mM PMSF