HelixAmp™ Premium-Pfu polymerase is an editorial enzyme improved from HelixAmp™ Speed-Pfu polymerase. HelixAmp™ Premium-Pfu polymerase shows faster (4 ~ 6 times) polymerization reaction and higher fidelity (3 ~ 5 times) than any other high-fidelity enzymes. With this high speed and accuracy, NanoHelix’s “PMT(polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. HelixAmp™ Premium-Pfu polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, the application of N-Solution™ improves the specificity and productivity of the reactions. 

Figure 1. Various annealing temperature of HelixAmp™ Premium-Pfu polymerase. 12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under the various annealing temperature. AT = annealing temperature.

Figure 2. Express speed of HelixAmp™ Premium-Pfu polymerase. (A): Premium-Pfu, (B): Power-Pfu. Four different size of DNA fragments were amplified with various extension times. PCR reactions were carried out 35 cycles of 10 sec at 95˚C and each represented extension time at 68˚C. These results are indicate that 60 sec of extension is enough for successful synthesis of 4 kb DNA fragment with HelixAmp™ Premium-Pfu polymerase. 

Application  

• High fidelity PCR 
• Long range PCR 
• Site-directed mutagenesis
• Blunt and PCR cloning

Contents  

• Premium-Taq polymerase (2.5 units/μl)
• 5x Reaction Mix [PP] (containing dNTPs)
• N-Solution™
• 6x Loading Dye 

Quality control 

• Contamination Assay : HelixAmp™ Premium-Pfu polymerase was passed from quality control assay for contamination of bacterial host DNA using sequence-specific primer set from host bacterial genomic DNA.
• Functioinal Assay : HelixAmp™ Premium-Pfu polymerase was functionally tested for PCR amplifications using the various primer sets (0.5 kb ~ 17 kb) from human beta-globin gene. 

Storage buffer 

• 50% Glycerol, 50 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Tween 20
• 0.1% Nonidet P-40, 1 mM PMSF