The RealHelix™ qPCR Kit [Probe] is designed to perform a rapid real-time quantification of target DNA using dual-labeled probes. The convenient 2x concentrated premix contains hot-start PCR enzyme, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the non-specific reactions and production of primer dimers. The separately supplied ROX Dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal. 

Human genomic DNAs (10, 1, 0.1, 0.01ng) isolated from whole blood were analyzed using Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is demonstrated by E=99.9%, R² value = 0.997. 20 µl reactions were operated on a CFX96 real-time PCR detection system (BioRad). 

Comparison of real-time PCR using RealHelix™ qPCR Kit [Probe] and the previous version (qPCR Kit [Probe type])

Human genomic DNAs (100, 10, 1, 0.1ng) isolated from whole blood were analyzed using beta-globin gene-specific primer set and 5’-Quasar705-labelled probe. Each 20 µl reactions were operated on a CFX96 real-time PCR detection system (BioRad).

RealHelix™ qPCR Kit [Probe] shows more reliable and efficient amplifications. 

Contents 

• 2x Premix [Probe]
• ROX Dye (25μM) 

Application 

• Real-time quantification of multiple target DNAs 
• Dual-labeled probe-based assays including TaqMan, Beacon, and Scorpions 
• Gene expression analysis of cDNA
• SNP analysis and Genotyping
• Microbial & viral pathogen detection
• Molecular diagnosis 

Quality control

RealHelix™ qPCR Kit [Probe] was evaluated by real-time PCR using the 10-fold serial-diluted human genomic DNA and a set of human gene-specific primer and

dual-labeled probe with ABI 7500 real-time PCR system (Applied Biosystems, USA) and Rotor-Gene Q instrument (QIAGEN, Germany).